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KMID : 0359319920320040555
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Korean Journal of Veterinary Research
1992 Volume.32 No. 4 p.555 ~ p.567
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Cloning and sequencing of the gene encoding structural protein of canine parvovirus isolated in Korea
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박종현/Park, Jong Hyeon
송재영/안수환/전무형/이중복/현방훈/Song, Jae Young/An, Soo Hwan/Jun, Moo Hyung/Lee, Jung Bok/Hyun, Bang Hun
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Abstract
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In this study gene encoding structural proteins of a CPV isolate was cloned and sequenced to elucidate the molecular genetical properties of the canine parvoviruses isolated from the field. Six recombinant plasmids of pEP3, p1471, p2070, pEP069, pEP338 and p14711p were constructed from the map positions 22 to 98 of RF DNA to clone the VP1 and VP2 genes of CPV-V20. Sequentialy the gene comprising 3780 nucleotides were sequenced by dideoxy chain termination method. When nucleotide sequence of gene encoding the structural proteins of CPV-V20 was compared with those of other strains, CPV-N, CPV-d and CPV-780929 published previously, DNP, homologies to CPV-V20 were 99.87% with CPV-N, 99.73% with CPV-d, 96.85% with CPV-780929 and 98.4% with FPLV-Carl, respectively. The DNA sequence data of CPV-V20 showed seven point mutations and also deletion of 13o nucleotides from the nucleotide position 4745 to 4879 located in the 3´-noncoding region of CPV-N.
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KEYWORD
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